The Ukrainian Museum preserves and interprets art and artifacts relating to the history and culture of this Eastern European nation. The museum's collection is divided into the areas of folk art, fine art and historical archive. Examples of folk art include wedding and festival attire; ritual cloths (rushnyky); kilims and other examples of embroidered textiles; ceramics; metalwork; jewelry; decorative wood objects and the ornate Easter eggs known as pysanky. Fine arts holdings include works by Ukrainian and Ukrainian-American artists of international importance, including painter Alexander Archipenko, sculptor Chreshnovsky and architect Vasyl Krychevsky.

    The archive contains photographs, posters, flyers, playbills and other artifacts relating to the life, history and cultural of Ukraine. Exhibitions have included The Lost Architecture of Kiev, a photographic survey of the many churches and other architectural landmarks demolished under the Soviet regime; and To Preserve a Heritage: Ukrainian Immigration to the United States. Courses in embroidery and gerdany (traditional beadwork) are offered, as are courses on pysanka making and traditional baking. Lectures, gallery tours and film screenings augment these activities. The museum was established in 1976 by the Ukrainian National Women's League of America.

    In 2005, the museum moved to a new 25,000-square-foot space on East Sixth Street in the East Village, which includes new features such as modern exhibition galleries; rooms for classes and workshops; a research library gallery/auditorium; space for conferences, lectures, slide and film presentations; a gift shop; and a café.